Gio Calixtro is 23 years old and was born in Cornelius, Oregon. He was part of the Westside Timbers before joining the Portland Timbers youth academy. He made his pro debut for Portland Timbers 2, a part of the USL Championship, on June 11, 2017. There, he scored 9 goals in 55 appearances and became the youngest player in tier 2 history to score 2 goals in a game (18 years, 9 months, 3 days).
In 2021, he signed with USL League One team FC Tuscon. There he had 5 goals in 50 appearances.
In July of 2023, Gio joined One Knoxville!